|
| Status |
Public on May 28, 2009 |
| Title |
Expression analysis upon NAMPT knockdown in MCF-7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
NAMPT is an enzyme in the mammalian NAD+ salvage pathway. Expression microarray analysis was used to study the effect of NAMPT knockdown on gene expression in MCF-7 breast cancer cells.
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| |
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| Overall design |
Stable knockdown of NAMPT was achieved using a retroviral shRNA construct. An shRNA directed against Luciferase was used to generate the Luc control cells. Three independent biological replicates with matching Luc controls were analyzed using Affymetrix U133 A2.0 microarrays.
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| |
|
| Contributor(s) |
Zhang T, Kraus WL |
| Citation(s) |
19478080 |
| Submission date |
Nov 03, 2008 |
| Last update date |
Dec 06, 2018 |
| Contact name |
W. Lee Kraus |
| E-mail(s) |
lee.kraus@utsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8511 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (6)
|
| GSM339268 |
MCF-7 cells_luciferase matching NAMPT_replicate 1 |
| GSM339269 |
MCF-7 cells_luciferase matching NAMPT_replicate 2 |
| GSM339270 |
MCF-7 cells_luciferase matching NAMPT_replicate 3 |
| GSM339271 |
MCF-7 cells_NAMPT knockdown_replicate 1 |
| GSM339272 |
MCF-7 cells_NAMPT knockdown_replicate 2 |
| GSM339273 |
MCF-7 cells_NAMPT knockdown_replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE13577 |
SIRT1-Dependent Gene Regulation Through Promoter-Directed Recruitment of a Nuclear NAD+ Synthase |
|
| Relations |
| BioProject |
PRJNA113977 |
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