|
| Status |
Public on Dec 05, 2008 |
| Title |
GM12146, Technical replicate |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid Cell Lines (LCL), CEU, GM12146
|
| Organism |
Homo sapiens |
| Characteristics |
HapMap CEU
|
| Growth protocol |
The cells were grown at a density of 5 x 10^5 mL in a humidified, 5% C02 incubator at 37 C in RPMI 1640 supplemented with 15% FBS, 2mM L-glutamine, 100 U/mL penicillin, and 0.1 mg/mL streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RNeasy Mini-Kit (Qiagen), reverse-transcribed using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
IVT was carried out with the BioArray High Yield T7 RNA transcript labeling kit (Enzo)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 46 C on HG-FOCUS Array. Arrays were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
HG-Fous arrays were scanned using the Affymetrix GS-2500 scanner.
|
| Description |
GM12146, Technical replicate
|
| Data processing |
The expression data was analyzed with Affymetrix MAS 5.0, scaled to 500 and log2 transformed. Further analysis was performed on genes defined as ‘Present’ in at least 80% of the individuals screened from one population.
|
| |
|
| Submission date |
Mar 14, 2008 |
| Last update date |
Nov 26, 2008 |
| Contact name |
Dustin Hancks |
| E-mail(s) |
hancks@mail.med.upenn.edu
|
| Phone |
215-898-2080
|
| Organization name |
University of Pennsylvania
|
| Department |
Genetics
|
| Lab |
Kazazian
|
| Street address |
415 curie blvd rm 515 crb
|
| City |
philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL201 |
| Series (1) |
| GSE10824 |
Gene expression levels in African Americans vary with genetic ancestry at most expressed genes |
|
