|
| Status |
Public on Mar 27, 2014 |
| Title |
Jurkat WT-MSCV K13-ER-TAM-treated with 4OHT |
| Sample type |
RNA |
| |
|
| Source name |
Jurkat WT-MSCV K13-ER-TAM-treated with 4OHT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Jurkat (BCBL1)
cell type: Leukemia
|
| Biomaterial provider |
Jurkat cells from Dr Shao-Cong Sun (Penn State University).
|
| Treatment protocol |
Cells were grown in presence or absence of 4OHT.
|
| Growth protocol |
Jurkat cells were grown in RPMI medium containing 10% FBS (fetal bovine serum) . Cells were stably transduced with either a MSCVneo vector or MSCVneo expressing a 4-Hydroxytamoxifen (4-OHT)-inducible K13-ER-TAM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the cells using RNeasy mini kit (Qiagen Valenica, CA)
|
| Label |
biotin
|
| Label protocol |
Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16hr on Affymetrix GeneChip U133A 2.0 Array as recommended by manufacturer
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Data processing |
The data were analyzed with Gene Spring GX 11.5.1.
|
| |
|
| Submission date |
Jul 26, 2013 |
| Last update date |
Mar 27, 2014 |
| Contact name |
Vasu Punj |
| E-mail(s) |
vasupunj@gmail.com
|
| Organization name |
USC
|
| Street address |
Biggy Street
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE49245 |
NF-kB essential modulator (NEMO) is essential for KSHV-encoded viral FLICE inhibitory protein (vFLIP) K13- induced gene expression and its N-terminal 251 resdidues are sufficent for this process |
|
