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| Status |
Public on Mar 27, 2014 |
| Title |
NF-kB essential modulator (NEMO) is essential for KSHV-encoded viral FLICE inhibitory protein (vFLIP) K13- induced gene expression and its N-terminal 251 resdidues are sufficent for this process |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We report here that KSHV viral infection targets the NF-kB pathway which is crucial for cell survival. KSHV protein vFLIP K13 is known to directly interact with cellular protein NEMO of the NF-kB pathway. We used gene expression array to suggets that the interaction of K13 with NEMO is important to activate NF-kB pathway.
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| Overall design |
We employed gene array analysis followed by bioinformatics analyses as well as biological pathways to determine the effect of K13 interaction with Nemo in Jurkat cell line system. Total RNA was isolated isolated using standard RNAeasy kit protocol (Qiagen). Their gene expression profiles were obtained using the Affymetrix Human Genome U133A 2.0 Array.
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| Contributor(s) |
Tolani B, Matta H, Gopalakrishnan R, Punj V, Chaudhary PM |
| Citation(s) |
24672029 |
| Submission date |
Jul 26, 2013 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Vasu Punj |
| E-mail(s) |
vasupunj@gmail.com
|
| Organization name |
USC
|
| Street address |
Biggy Street
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (8)
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| GSM1195871 |
Jurkat WT-MSCV K13-ER-TAM-treated with 4OHT |
| GSM1195872 |
Jurkat Nemo deficent-MSCV-vector-untreated |
| GSM1195873 |
Jurkat Nemo deficent-MSCV-vector-treated with 4OHT |
| GSM1195874 |
Jurkat Nemo deificent-MSCV K13-ER-TAM-untreated |
| GSM1195875 |
Jurkat Nemo deificent-MSCV K13-ER-TAM-treated with 4OHT |
|
| Relations |
| BioProject |
PRJNA213382 |
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