|
| Status |
Public on Mar 22, 2017 |
| Title |
Midgut transcriptome of Culex quinquefasciatus larvae treated with LC90 doses of Bin toxin from Lysinibacillus sphaericus |
| Organism |
Culex quinquefasciatus |
| Experiment type |
Expression profiling by array
|
| Summary |
Lysinibacillus sphaericus produces the mosquito larvicidal binary toxin consisting of BinA and BinB, which are both required for toxicity against Culex and Anopheles larvae. The molecular mechanisms behind Bin toxin-induced damage remain unexplored. We used whole-genome microarray-based transcriptome analysis to better understand how Culex larvae respond to Bin toxin treatment at the molecular level. Our analyses of Culex quinquefasciatus larvae transcriptome changes at 6, 12, and 18 h after Bin toxin treatment revealed a wide range of transcript signatures, including genes linked to the cytoskeleton, metabolism, immunity, and cellular stress, with a greater number of down-regulated genes than up-regulated genes. Bin toxin appears to mainly repress the expression of genes involved in metabolism, the mitochondrial electron transport chain, and the protein transporter of the outer/inner mitochondrial membrane. The induced genes encode proteins linked to mitochondrial-mediated apoptosis and cellular detoxification including autophagic processes and lysosomal compartments.
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| Overall design |
Midgut of untreated control vs. Bin toxin treated Culex quinquefasciatus larvae 4 biological replicates each
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| Contributor(s) |
Tangsongcharoen C, Jupatanakul N, Promdonkoy B, Dimopoulos G, Boonserm P |
| Citation(s) |
28406958 |
| Submission date |
Mar 21, 2017 |
| Last update date |
Mar 11, 2020 |
| Contact name |
George Dimopoulos |
| E-mail(s) |
gdimopo1@jhu.edu
|
| Phone |
4432870128
|
| Organization name |
Johns Hopkins School of Public Health
|
| Department |
Molecular Microbiology and Immunology
|
| Street address |
615 N Wolfe St.
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10712 |
Culex quinquefasciatus genome array annotation 1 |
|
| Samples (12)
|
| GSM2544876 |
Untreated vs toxin treated midgut 6hr 1 |
| GSM2544877 |
Untreated vs toxin treated midgut 6hr 2 |
| GSM2544878 |
Untreated vs toxin treated midgut 6hr 3 |
| GSM2544879 |
Untreated vs toxin treated midgut 6hr 4 |
| GSM2544880 |
Untreated vs toxin treated midgut 12hr 1 |
| GSM2544881 |
Untreated vs toxin treated midgut 12hr 2 |
| GSM2544882 |
Untreated vs toxin treated midgut 12hr 3 |
| GSM2544883 |
Untreated vs toxin treated midgut 12hr 4 |
| GSM2544884 |
Untreated vs toxin treated midgut 18hr 1 |
| GSM2544885 |
Untreated vs toxin treated midgut 18hr 2 |
| GSM2544886 |
Untreated vs toxin treated midgut 18hr 3 |
| GSM2544887 |
Untreated vs toxin treated midgut 18hr 4 |
|
| Relations |
| BioProject |
PRJNA379909 |
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