GSM4223463: CAGAGAGGTTACAT_Lane1_Ctrl_Tfh2; Mus musculus; RNA-Seq - SRA

SRX7400806: GSM4223463: CAGAGAGGTTACAT_Lane1_Ctrl_Tfh2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 1500) run: 8.1M spots, 602.4M bases, 391.3Mb downloads

Submitted by: NCBI (GEO)

Study: Continued Bcl6 expression prevents the transdifferentiation of established Tfh cells into Th1 cells during acute viral infection

PRJNA596262 • SRP238013 • All experiments • All runs

show Abstracthide Abstract

By using a temporally-controlled system for the ablation of Bcl6 and Cxcr5 speficially in CD4 T cells, the requirements of both factors for the maintenance of Tfh cells were investigated Overall design: Cd4-CreERT2+ mice without floxed alleles were used as controls in all experiments. Bcl6 ablation in SMARTA cells in LCMV infection: iBcl6KO (n=5), Ctrls (n=3) Cxcr5 ablation in SMARTA cells in LCMV infection: iCxcr5KO (Tfh: n=4, Th1: n=4), Ctels (Tfh: n=3; Th1: n=5) Cxcr5 ablation in NP-KLH immunization: iCxcr5KO (Tfh: n=6), Ctrls (Tfh: n=4; non-Tfh: n=4)

Sample: CAGAGAGGTTACAT_Lane1_Ctrl_Tfh2

SAMN13616395 • SRS5848467 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 1500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: secondary lymphoid organs were removed, single cell suspensions and antibody stainings for flow cytometry and cell sorting were prepared on ice. Cells were sorted directly into lysis buffer and immediately spun down and frozen on dry ice. RNA was isolated using magnetic beads Libraries were constructed using a bulk adjusted mcSCRB-seq protocol.

Experiment attributes:

GEO Accession: GSM4223463

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 8.1M spots, 602.4M bases, 391.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR10724671	8,140,659	602.4M	391.3Mb	2020-10-06

ID:: 9687068

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