GSM3910582: CHO cells, A03_EXP_Duplicate; Cricetulus griseus; RNA-Seq - SRA

SRX6384007: GSM3910582: CHO cells, A03_EXP_Duplicate; Cricetulus griseus; RNA-Seq
4 ILLUMINA (Illumina MiSeq) runs: 15.9M spots, 1.2G bases, 436.1Mb downloads

Submitted by: NCBI (GEO)

Study: Transcriptome profiles of antibody-producing CHO cells across different amplification strategies

PRJNA551666 • SRP212617 • All experiments • All runs

show Abstracthide Abstract

The mechanism of action behind the increased secretion capacity of the amplified progeny of CHO cells is investigated in this study through comparative transcriptomic analysis Overall design: The amplified DG-44 CHO cells, taken at both exponential and stationary growth phase were compared to non-amplified CHO DG-44 cells. Non-producing CHO-S samples were used as negative controls.

Sample: CHO cells, A03_EXP_Duplicate

SAMN12162719 • SRS5043525 • All experiments • All runs

Organism: Cricetulus griseus

Library:

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cell cultures were re-suspended in RLT buffer (Qiagen) and kept at −80 °C until RNA was extracted using the RNeasy kit (Qiagen) and on-column DNAse digestion. Library preparation was performed with Illumina's TruSeq Stranded mRNA Library Prep Kit High Throughput (Catalog ID: RS-122-2103), according to manufacturer's protocol

Experiment attributes:

GEO Accession: GSM3910582

Links:

NCBI link: NCBI Entrez (gds)

Runs: 4 runs, 15.9M spots, 1.2G bases, 436.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR9621007	3,972,963	299.7M	108.8Mb	2019-10-01
SRR9621008	3,888,866	293.4M	105.9Mb	2019-10-01
SRR9621009	4,026,429	303.7M	110.4Mb	2019-10-01
SRR9621010	4,024,051	303.6M	111Mb	2019-10-01

ID:: 8457075

SRA

Sequence Read Archive

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