GSM3910579: CHO cells, A02_EXP; Cricetulus griseus; RNA-Seq - SRA

SRX6384004: GSM3910579: CHO cells, A02_EXP; Cricetulus griseus; RNA-Seq
4 ILLUMINA (Illumina MiSeq) runs: 18.4M spots, 1.4G bases, 505.6Mb downloads

Submitted by: NCBI (GEO)

Study: Transcriptome profiles of antibody-producing CHO cells across different amplification strategies

PRJNA551666 • SRP212617 • All experiments • All runs

show Abstracthide Abstract

The mechanism of action behind the increased secretion capacity of the amplified progeny of CHO cells is investigated in this study through comparative transcriptomic analysis Overall design: The amplified DG-44 CHO cells, taken at both exponential and stationary growth phase were compared to non-amplified CHO DG-44 cells. Non-producing CHO-S samples were used as negative controls.

Sample: CHO cells, A02_EXP

SAMN12162722 • SRS5043522 • All experiments • All runs

Organism: Cricetulus griseus

Library:

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cell cultures were re-suspended in RLT buffer (Qiagen) and kept at −80 °C until RNA was extracted using the RNeasy kit (Qiagen) and on-column DNAse digestion. Library preparation was performed with Illumina's TruSeq Stranded mRNA Library Prep Kit High Throughput (Catalog ID: RS-122-2103), according to manufacturer's protocol

Experiment attributes:

GEO Accession: GSM3910579

Links:

NCBI link: NCBI Entrez (gds)

Runs: 4 runs, 18.4M spots, 1.4G bases, 505.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR9620995	4,609,605	347.7M	126.3Mb	2019-10-01
SRR9620996	4,518,089	340.8M	123Mb	2019-10-01
SRR9620997	4,658,823	351.4M	127.7Mb	2019-10-01
SRR9620998	4,659,618	351.5M	128.5Mb	2019-10-01

ID:: 8457072

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity