GSM3450466: Column_15_Estrogen; Mus musculus; RNA-Seq - SRA

SRX4954320: GSM3450466: Column_15_Estrogen; Mus musculus; RNA-Seq
4 ILLUMINA (NextSeq 500) runs: 6.7M spots, 675.4M bases, 279Mb downloads

Submitted by: NCBI (GEO)

Study: Single-cell RNA sequencing reveals transcriptional dynamics of estrogen-induced dysplasia in the ovarian surface epithelium

PRJNA501791 • SRP167204 • All experiments • All runs

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We use single-cell RNA sequencing (scRNA-seq) to explore the transcriptional changes associated with estrogen-induced dysplasia in mouse ovarian surface epithelial cells Overall design: scRNA-seq of control and estrogen-treated (100nM) mOSE cultured for 15 days. scRNA-seq was performed using the Fluidigm HT 3' RNA-seq protocol on the Fluidigm C1

Sample: Column_15_Estrogen

SAMN10345580 • SRS3995710 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Single cell suspensions of control and E2-treated mOSE were processed using the Fluidigm HT 3' scRNA-seq protocol. The left cell inlet of the integrated fluidics circuit (IFC; leading to columns 1-10 of the plate) were loaded with control cells, while E2-treated cells were loaded into the right inlet (columns 11-20 of the IFC plate) Library were constructed using the standard protocol described for the Fluidigm HT 3' scRNA-seq. Libraries were sequenced on a NextSeq500 high-out 26x75bp run

Experiment attributes:

GEO Accession: GSM3450466

Links:

NCBI link: NCBI Entrez (gds)

Runs: 4 runs, 6.7M spots, 675.4M bases, 279Mb

Run	# of Spots	# of Bases	Size	Published
SRR8133274	2,029,620	204.3M	82.7Mb	2018-11-02
SRR8133275	1,710,865	172.2M	71.5Mb	2018-11-02
SRR8133276	1,539,983	155M	63.9Mb	2018-11-02
SRR8133277	1,429,647	143.9M	60.9Mb	2018-11-02

ID:: 6682496

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