GSM3369696: WT_exp_2; Aspergillus niger; RNA-Seq - SRA

SRX4628185: GSM3369696: WT_exp_2; Aspergillus niger; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 17.9M spots, 5.4G bases, 2.1Gb downloads

Submitted by: NCBI (GEO)

Study: Comparative transcriptomics of Aspergillus niger when deleted or overexpressed for the putative transcription factors MjkA, MjkB and the histon deactelyase HdaX

PRJNA488723 • SRP159238 • All experiments • All runs

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Purpose: Expression profiling of two ORFs encoding putative transcription factors: An07g07370 (TF1/MjkA) and An12g07690 (TF2/MjkB), and a histone deacetylase (An09g06520, HdaX) under carbon-limited batch cultivations (biological duplicate runs) in Aspergillus niger. Methods: Single deletion strains for TF1, TF2 and HD, respectively, (ii) a double deletion strain for TF1 and TF2, and (iii) individual conditional overexpression mutants for TF1, TF2 and HD using the Tet-on system were analysed. Samples were taken at exponential and post-exponential growth phase (technical duplicate) and analysed by RNA-Seq analysis (DOI). Overall design: mRNA profiles of MjkA and MjkB transcription factors deletion and over expression strains in A. niger in duplicates, Illumina HiSeq

Sample: WT_exp_2

SAMN09940777 • SRS3729479 • All experiments • All runs

Organism: Aspergillus niger

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Mycelium was sampled, flash frozen and grinded. RNA was extracted using TRIzol method from Vitrogen RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM3369696

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 17.9M spots, 5.4G bases, 2.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR7772838	17,920,768	5.4G	2.1Gb	2018-11-07

ID:: 6253054

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