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SRX19733324: GSM7107735: sir4∆_wtpromoter_Sua7FLAG input rep1; Saccharomyces cerevisiae; ChIP-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 3M spots, 310M bases, 96.1Mb downloads

External Id: GSM7107735_r1
Submitted by: Rine, Molecular and Cell Biology, University of California, Berkeley
Study: Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIP-seq III]
PRJNA946783 • SRP428314 • All experiments • All runs
show Abstracthide Abstract
As part of a study on the mechanism of Sir-based silencing at HML in S.cerevisiae, and the contribution of Rap1 to transcription of the locus, we performed ChIP-seq of multiple components of RNA Pol II machinery in different genetic backgrounds including: sir4?, SIR+, and with or without a mutation that abbrogated Rap1 binding at the alpha promoter. Overall design: Taf1-3xFLAG ChIPseq; Sua7-3xFLAG ChIPseq; Elf1-3xFLAG ChIPseq; Rpb3-3xFLAG ChIPseq in silenced (SIR) or unsilenced (sir4?) cells, with or without a mutation in the alpha2 promoter.
Sample: sir4∆_wtpromoter_Sua7FLAG input rep1
SAMN33831998 • SRS17100097 • All experiments • All runs
Organism: Saccharomyces cerevisiae
Library:
Name: GSM7107735
Instrument: Illumina NovaSeq 6000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: Cells were lysed by mechanical disruption in a bead beater. Chromatin was fragmented by sonication. IPs were performed using 5mL mouse monoclonal anti-FLAG® M2 antibody (MilliporeSigma, product no F1804) and 50uL DynaBeads Protein G magnetic beads (ThermoFisher Scientific catalog number 10003D). DNA was purified using Qiagen spin column kit NEB Ultra II library prep kit
Runs: 1 run, 3M spots, 310M bases, 96.1Mb
Run# of Spots# of BasesSizePublished
SRR239227053,039,190310M96.1Mb2023-05-09

ID:
27044309

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