GSM5884346: LAN326A11_S6_L003; Mus musculus; RNA-Seq - SRA

SRX14121161: GSM5884346: LAN326A11_S6_L003; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 17.7M spots, 1.3G bases, 424.8Mb downloads

External Id: GSM5884346_r1

Submitted by: Francis Crick Institute

Study: Dyrk1a dosage effects on the transcriptome of embryonic hearts from the Dp1Tyb mouse model of Down syndrome using bulk RNAseq

PRJNA804913 • SRP359091 • All experiments • All runs

show Abstracthide Abstract

We performed bulk RNAseq on whole hearts from E13.5 Dp1Tyb embryos and from wild-type littermates, as well as hearts from E13.5 Dp1TybDyrk1a+/+/- embryos and wild-type littermates. Analysis showed the expected increased expression of the Hsa21-orthologous genes on Mmu16 that are present in 3 copies. Gene set enrichment analysis identified pathways that are altered in Dp1Tyb hearts. Some of these changes were dependent on 3 copies of Dyrk1a. Overall design: RNAseq on 5 E13.5 Dp1Tyb embryonic hearts and 5 wild-type littermate embryonic hearts, and on 5 E13.5 Dp1TybDyrk1a+/+/- embryonic hearts and 5 wild-type littermate embryonic hearts.

Sample: LAN326A11_S6_L003

SAMN25816643 • SRS11943992 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM5884346

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Hearts were dissected and snap frozen and kept at -80C. Samples were lysed (buffer RLT Qiagen RNeasy Minikit) and homogenised using a battery-operated pestle motor mixer followed by RNA extraction using Qiagen RNeasy Mini kit using the standard protocol. cDNA library synthesis using KAPA mRNA HyperPrep Kit (Illumina) and libraries were sequenced on the Illumina HiSeq 2500 following manufacturer's protocols.

Runs: 1 run, 17.7M spots, 1.3G bases, 424.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR17964819	17,738,140	1.3G	424.8Mb	2024-01-26

ID:: 19895106

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity