Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Fat harvested and enzymatically processed per Cytori protocol to isolate a stromal based fraction of cells, frozen, thawed, DAPI excluded to select live cells RNA isolation based on Chromium Next GEM Single Cell 3' v3 kit, standard Illumina PolyA selection library prep before NextSeq500 sequencing