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SRX23668402: Whole genome sequencing of Vibrio fischeri strain derived from ES114
1 ILLUMINA (NextSeq 2000) run: 1.4M spots, 405M bases, 140.1Mb downloads

Design: Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.
Submitted by: University of Georgia
Study: Vibrio fischeri D-alanine suppressor generation and characterization
PRJNA1078398 • SRP490497 • All experiments • All runs
show Abstracthide Abstract
This study used D-alanine auxotrophic mutants of Vibrio fischeri to study the evolution of peptidoglycan biosynthesis and structure. Suppressor mutants were generated by growing an auxotrophic strain without necessary exogenous D-alanine. Mutants were then characterized and genome sequenced, in order to determine the mechanism of suppression.
Sample:
SAMN39999386 • SRS20499473 • All experiments • All runs
Organism: Aliivibrio fischeri
Library:
Name: MC6S08
Instrument: NextSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 1.4M spots, 405M bases, 140.1Mb
Run# of Spots# of BasesSizePublished
SRR280162541,362,417405M140.1Mb2024-02-20

ID:
31951819

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