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SRX21649989: GSM7764482: 80hpf; Danio rerio; RNA-Seq
1 ILLUMINA (NextSeq 2000) run: 225.3M spots, 18.8G bases, 8.3Gb downloads

External Id: GSM7764482_r1
Submitted by: Bioinformatics, Max-Planck-Institute for Heart and Lung Research
Study: scRNA-seq of 50 and 80 hpf isolated zebrafish hearts
PRJNA1013567 • SRP458853 • All experiments • All runs
show Abstracthide Abstract
Seeking to identify additional transcription factors required for cardiac valve formation, we determined and explored the transcriptional landscape of endocardial cells at the time when key morphogenetic events underlying valve development take place. Overall design: We isolated wild-type zebrafish hearts at 50 hours post-fertilization (hpf) when valve identity has just been established, and at 80 hpf when forming valves are first observed
Sample: 80hpf
SAMN37304285 • SRS18818461 • All experiments • All runs
Organism: Danio rerio
Library:
Name: GSM7764482
Instrument: NextSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: PAIRED
Construction protocol: Whole hearts were isolated and cardiac cells were dissociated. Dead cells were removed from the final cell isolate by FACs sorting in PBS without Calcium and Magnesium and with 0.04% BSA. Each sample was run separately on a lane in a Chromium controller with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3,1 (10xGenomics). scRNA-seq library preparation was done using a standard protocol, and sequencing was done on a Nextseq2000
Runs: 1 run, 225.3M spots, 18.8G bases, 8.3Gb
Run# of Spots# of BasesSizePublished
SRR25930974225,273,32418.8G8.3Gb2024-02-13

ID:
29305547

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