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SRX13242203: GSM5705746: W_hira_3; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 26.5M spots, 8G bases, 2.3Gb downloads

Submitted by: NCBI (GEO)
Study: Epigenetic reprogramming orchestrates transcriptional responses in shade avoidance syndrome of Arabidopsis
show Abstracthide Abstract
For shade-intolerant plants, changes in light quality indicative of competition from neighboring plants trigger shade avoidance syndrome (SAS). PYHTOCHROME-INTERACTING FACTOR 7 is the major transcriptional regulator of SAS in Arabidopsis. However, the epigenetic reprogramming under shade is poorly understood. To identify the histone chaperone ASF1 and HIRA function during SAS, we performed transcriptome deep sequencing (RNA-seq) to search for differentially expressed genes (DEGs) by comparing transcript levels between Col-0 and pif7-1, asf1ab, or hira-1 seedlings during white light and shade conditions. Our data shown that histone chaperone ASF1, through interacting with PIF7 and helping of HIRA, positively regulates shade-induced genes expression. Overall design: 6-day-white-light grown seedlings were treated with 1 hr shade before harvesting for RNA-seq.
Sample: W_hira_3
SAMN23470232 • SRS11166407 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from the snap-frozen tissues using TRIzol® Reagent (Invitrogen) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5705746
Links:
Runs: 1 run, 26.5M spots, 8G bases, 2.3Gb
Run# of Spots# of BasesSizePublished
SRR1705296426,494,0658G2.3Gb2023-01-31

ID:
18114112

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