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SRX10016438: GSM5062127: 0x_B; Perkinsus marinus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 71.2M spots, 21.5G bases, 7.1Gb downloads

Submitted by: NCBI (GEO)
Study: A dual omics approach to evaluate transcriptional and metabolic responses during lipid deprivation in an oyster parasite Perkinsus marinus
show Abstracthide Abstract
In this study, we explored the metabolic processes of P. marinus under lipid deprived conditions to elucidate the interchanging flux of lipid and carbohydrate metabolism. Though P. marinus can synthesize their own lipids from available nutrients, they display a slower growth in media not supplemented with lipids as opposed to media with lipids. Under these conditions, using transcriptomics, we surprisingly observed evidence of stimulated lipid degradation through increased transcription of two core ß-oxidation pathway enzymes. Overall design: We sought to examine the transciptional effects of lipid withdrawal from growth media using RNA seq methodologies.
Sample: 0x_B
SAMN17773440 • SRS8184744 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: P. marinus cells were harvested from day 11 lipid replete and lipid deplete media for each biological replicate. Cells were pelleted at 3,900 RPM for 10 minutes at 4 C° using an Eppendorf 5810R Centrifuge. P. marinus total RNA was extracted per manufacturer's protocol using the Qiagen RNeasy mini- kit; RNA quality was verified on a 1% percent agarose gel and visualized using a UV transilluminator. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM5062127
Links:
Runs: 1 run, 71.2M spots, 21.5G bases, 7.1Gb
Run# of Spots# of BasesSizePublished
SRR1362335371,184,23721.5G7.1Gb2021-02-05

ID:
13143852

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