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SRX9418436: GSM4876567: HeLa_4sU_empty_rep2; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 46.7M spots, 4.8G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: A first exon termination checkpoint that preferentially suppresses extragenic transcription [RNASEQ_ZC3H4_C]
PRJNA673870 • SRP290828 • All experiments • All runs
show Abstracthide Abstract
Wdr82 and Zc3h4 repress transcription at extragenic sites. Overall design: Transcriptome analysis to measure transcription in cells depleted of Wdr82, Zc3h4or other known termination factors.
Sample: HeLa_4sU_empty_rep2
SAMN16624780 • SRS7633781 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted using Trizol (Thermofisher cat. 15596026); 4sU-labelled RNA was extracted as described (Austenaa et al. 2015, PMID 26593720) Library preparation was performed using the TruSeq Stranded Total RNA sample preparation kit (Illumina RS-122-9007) starting from ca. 80-200 ng of nascent 4sU-labelled RNA, without ribosomal depletion.
Experiment attributes:
GEO Accession: GSM4876567
Links:
Runs: 1 run, 46.7M spots, 4.8G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR1296588346,714,4794.8G1.3Gb2021-02-01

ID:
12299634

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