RNA-seq of axillary buds: Switchgrass - SRA

SRX9038537: RNA-seq of axillary buds: Switchgrass
1 ILLUMINA (Illumina HiSeq 3000) run: 31.2M spots, 9G bases, 3.3Gb downloads

Design: We extracted RNAs from axillary buds of tillers. The mutant 52-1 with highly enhanced tiller production and the WT plant (WT-1) generated from the same callus line from which the mutant 52-1 was obtained were chosen for mRNA-seq. Three biological replications were sampled for each plant. All visible axillary buds collected from two to three tillers are treated as one biological replicate. Total RNA was isolated from tissue samples using the Qiagen RNeasy Plant Isolation kit according to the manufacturers protocol (Qiagen Inc., Valencia, CA, USA) and the RNA quality was checked using BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). cDNA libraries were constructed by the ISU DNA Facility according to the instructions in the Illumina sequencing manual.

Submitted by: Iowa State University

Study: Transcriptome sequencing of axillary buds of switchgrass Pvtb1 mutant and WT plant.

PRJNA660286 • SRP279368 • All experiments • All runs

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It was shown in the expression database that Pvtb1 genes in switchgrass expressed highly in axillary buds (Panicum virgatum v1.0, DOE-JGI, http://phytozome.jgi.doe.gov/). Thus, we extracted RNAs from axillary buds of tillers. The mutant 52-1 with highly enhanced tiller production and the WT plant (WT-1) generated from the same callus line from which the mutant 52-1 was obtained were chosen for mRNA-seq. RNA-seq analysis revealed a complex regulatory network potentially regulating tillering in switchgrass and provided some clues to the pathways of Pvtb1 genes.

Sample:

SAMN15944670 • SRS7288998 • All experiments • All runs

Organism: Panicum virgatum

Library:

Name: 52_1_3

Instrument: Illumina HiSeq 3000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 31.2M spots, 9G bases, 3.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR12549313	31,212,345	9G	3.3Gb	2020-08-30

ID:: 11745208

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