RNA-Seq of Hermertia illucens L: late pupa stage - SRA

SRX5060466: RNA-Seq of Hermertia illucens L: late pupa stage
1 ILLUMINA (Illumina HiSeq 2000) run: 31.8M spots, 9.5G bases, 3.4Gb downloads

Design: After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using Illumina HiSeqXTen.

Submitted by: Huazhong Agricultural University

Study: Transcriptome sequencing of black soldier fly

PRJNA506627 • SRP170683 • All experiments • All runs

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investigate the fat accumulation mechanism by transcriptome sequencing.

Sample: collected in late pupa stage

SAMN10465380 • SRS4076407 • All experiments • All runs

Organism: Hermetia illucens

Library:

Name: L-pupa_2

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 31.8M spots, 9.5G bases, 3.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR8242285	31,753,348	9.5G	3.4Gb	2020-11-01

ID:: 6829874

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Sequence Read Archive

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