RNAseq of n elephant seal blubber during repeated ACTH challenge - SRA

SRX4528812: RNAseq of n elephant seal blubber during repeated ACTH challenge
1 ILLUMINA (Illumina HiSeq 4000) run: 54.5M spots, 11G bases, 4Gb downloads

Design: cDNA library preparation and Illumina HiSeq4000 sequencing were conducted at the University of California, Berkeley QB3 Vincent J. Coates Genomics Sequencing Laboratory. Ribosomal RNA (rRNA) was depleted using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat; Illumina, Inc., USA). Yield and quality of mRNA were assessed using fluorometric methods and an Agilent 2100 Bioanalyzer, respectively (Agilent Technologies, USA). PrepX directional RNAseq library kits (WaferGen Bio-systems Inc., USA) were used for library preparation and libraries were quantified with KAPA Library Quantification kits for Illumina platforms (Kapa Biosystems, USA) using a Bio-Rad CFX Connect (Bio-Rad Laboratories, USA). Thirteen cycles of indexing PCR using KAPA High Fidelity Hotstart Amplification Kits (Kapa Biosystems, USA) were used before library quantification and validation. Individual libraries were barcoded, indexed as TruSeq single index per library, pooled in equimolar concentrations (400 ng), and sequenced as 100 base-pair paired-end reads on the Illumina HiSeq 4000.

Submitted by: University of the Pacific

Study: Mirounga angustirostris blubber transcriptome response to repeated ACTH administration

PRJNA485363 • SRP157071 • All experiments • All runs

show Abstracthide Abstract

Global gene expression (transcriptome) response of blubber tissue of northern elephant seals to repeated stress challenge (adrenocorticotropic hormone administration).

Sample: seal4_D4T4

SAMN09792029 • SRS3646675 • All experiments • All runs

Organism: Mirounga angustirostris

Library:

Name: seal4_D4T4

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Inverse rRNA

Layout: PAIRED

Runs: 1 run, 54.5M spots, 11G bases, 4Gb

Run	# of Spots	# of Bases	Size	Published
SRR7668299	54,459,381	11G	4Gb	2018-10-02

ID:: 6133629

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity