cDNA library of hemiscorpius lepturus venom gland - SRA

SRX1584448: cDNA library of hemiscorpius lepturus venom gland
1 ILLUMINA (Illumina HiSeq 2000) run: 171,976 spots, 5.3M bases, 2Mb downloads

Design: The mRNA isolated from venom gland of Iranian dangerous scorpion. The mRNA libraries were prepared in accordance to the Illumina TruSeq RNA sample preparation guide for Illumina Paired-End Multiplexed Sequencing. According to the Illumina mRNA libraries preparation protocol, poly-A mRNA in the tRNA samples were first purified using Illumina poly-T oligo-attached magnetic beads and two rounds of purification. During the second elution of the poly-A RNA, the mRNA were also fragmented and primed with random hexamers for cDNA synthesis. Cleaved mRNAs were reverse transcribed into first strand cDNA using reverse transcriptase and random primers. The RNA template was then removed and a replacement strand synthesized to generate double-stranded cDNA. ollowing the standard protocol, after the first and second strands cDNA synthesis, ends were repaired, dA base added, and Illumina Indexing adapters were ligated. Finally, cDNA fragments that have adapter molecules on both ends underwent 15 cycles of PCR to amplify the amount of prepared material. The following Adapters sequences were used for the libraries preparation and amplification.

Submitted by: Pasteur Institute of Iran

Study: Hemiscorpius lepturus Transcriptome or Gene expression

PRJNA312013 • SRP070907 • All experiments • All runs

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The cDNA library of scorpion venom gland prepared to study transcriptome of Hemiscorpius lepturus venom

Sample:

SAMN04492130 • SRS1299325 • All experiments • All runs

Organism: Hemiscorpius lepturus

Library:

Name: cDNA library of hemiscorpius lepturus venom gland

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Spot descriptor:

1 forward

Experiment attributes:

authors: Fatemeh Kazemi-Lomedasht, Kamran Pooshang Bagheri, (show full text...)(hide...)

Fatemeh Kazemi-Lomedasht, Kamran Pooshang Bagheri, Mahdi Behdani, Delavar Shahbazzadeh

Runs: 1 run, 171,976 spots, 5.3M bases, 2Mb

Run	# of Spots	# of Bases	Size	Published
SRR3168566	171,976	5.3M	2Mb	2016-05-14

ID:: 2238224

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