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SRX1516056: GSM2027334: Host-P3_0_rep3; Pseudomonas aeruginosa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 14.3M spots, 1.1G bases, 705Mb downloads

Submitted by: NCBI (GEO)
Study: Biological characterization of virulent therapeutic bacteriophage PAK_P3 infecting Pseudomonas aeruginosa.
PRJNA307603 • SRP068063 • All experiments • All runs
show Abstracthide Abstract
We have isolated and characterized several bacteriophages infecting Pseudomonas aeruginosa distantly related to Felix O1 virus and proposed they form a new subfamily named Felixounavirinae. The infectious cycle of bacteriophages belonging to this subfamily has not been studied yet in terms of gene expression. The present study reports the RNA-Seq analysis of bacteriophage PAK_P3 infecting PAK strain of P. aeruginosa. Overall design: RNA profile of Host and Phage at 0min, 3.5min and 13 min after infection of Pseudomonas aeruginosa PAK strain with the Pseudomonas phage PAK P3. Three biological replicates for each time point.
Sample: Host-P3_0_rep3
SAMN04383811 • SRS1234746 • All experiments • All runs
Organism: Pseudomonas aeruginosa PAK
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cells were then lyzed in TRIzol,® total RNA was purified through a standard organic extraction and ethanol precipitation followed by DNase treatment (TURBO DNase®) and rRNA depletion (Ribo-Zero rRNA Removal Kit) RNA libraries were prepared for sequencing using standard Illumina protocols Truseq stranded RNA seq kit
Experiment attributes:
GEO Accession: GSM2027334
Links:
Runs: 1 run, 14.3M spots, 1.1G bases, 705Mb
Run# of Spots# of BasesSizePublished
SRR308437514,346,7211.1G705Mb2016-07-25

ID:
2144154

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