RNAseq analysis of tumours from GEMMS animals to determine the effect... - SRA

ERX11798548: RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ
1 ILLUMINA (Illumina NovaSeq 6000) run: 33.5M spots, 10G bases, 3Gb downloads

Design: RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ

Submitted by: EBI (European Bioinformatics Institute)

Study: RNAseq analysis of tumours from GEMMS animals to determine the effect of different aPDL1 combinations on lung tumours in situ

PRJEB71814 • ERP156600 • All experiments • All runs

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This study aims to identify combination treatments capable of inducing improved IO responses in lung tumours and thus, help guide decisions on the next combination arms for the HUDSON trial (post-IO). For that purpose, a lung tumour GEMM model was treated with either vehicle, PD-L1, ATR, ATR/PD-L1; Cisplatin/PD-L1/Ctla4 or VEGFR/PD-L1 and tumours collected for transcriptional profiling.

Sample: LUGEMM14 5-38

SAMEA115098472 • ERS17733219 • All experiments • All runs

Organism: Mus musculus

Library:

Name: LUGEMM14 5-38_p

Instrument: Illumina NovaSeq 6000

Strategy: AMPLICON

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Construction protocol: Samples were collected from GEMMs lung lesions and assessed by MRI quantification of combined lung tumour lesions per animal before & after treatment RNA was extracted using the RNeasy Mini Kit (Qiagen; #74104). RNA concentration was determined by Qubit Flex Fluorometer (Invitrogen), RNA purity was determined using a NanoDrop Eight (Thermo Scientific) and RNA integrity was measured using a 4200 Tapestation (Agilent) Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs; E7760L) or NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs; E7770L) as per manufactuer's guidelines. Ribosomal RNA was removed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs; E7490L) .

Runs: 1 run, 33.5M spots, 10G bases, 3Gb

Run	# of Spots	# of Bases	Size	Published
ERR12422282	33,474,838	10G	3Gb	2024-01-30

ID:: 31670698

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