Illumina HiSeq 2500 paired end sequencing of SAMD00092814 - SRA

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DRX189353: Illumina HiSeq 2500 paired end sequencing of SAMD00092814
1 ILLUMINA (Illumina HiSeq 2500) run: 19.4M spots, 3.9G bases, 2.2Gb downloads

Submitted by: OKA_IPSR

Study: Transcriptome analysis of infection process of Heterosigma akashiwo virus strain 53

PRJDB6241 • DRP007783 • All experiments • All runs

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Heterosigma akashiwo virus (HaV) is a new member of Phycodnaviridae, that possesses large double stranded DNA genome. Comparative analysis of its genome and other large dsDNA viruses revealed that HaV is unique new member of the viral family. To understand its biological feature more in detail, we conducted transcriptome analysis of the viral infection process employing different types of RNAseq approaches.

Sample: Heterosigma akashiow strain H93616,Heterosigma akashiwo virus strain 53-infected,90 mpi,replicate,2,ribosomal RNA-depleted

SAMD00092814 • DRS206853 • All experiments • All runs

Organism: Heterosigma akashiwo

Library:

Name: H93616_RiboZero_90mpi_2

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Inverse rRNA

Layout: PAIRED

Construction protocol: Total RNA was prepared usingPureLink RNA purification kit (Ambion)reagent according to the manufacturer's protocol. The ribosomal RNA was removed by using RiboZero Plant rRNA removal kit (Illumina) . The sequence library was prepared according to the Agilent SureSelect Strand Specific RNA Prep Kit protocol (skip the PolyA selection step).

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 19.4M spots, 3.9G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
DRR198936	19,396,653	3.9G	2.2Gb	2021-10-16

ID:: 17174752

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