Protein Family Models

This winged helix domain is found in the DprA protein [1]. [1]. 25138221. Roles of Bacillus subtilis DprA and SsbA in RecA-mediated. genetic recombination.. Yadav T, Carrasco B, Serrano E, Alonso JC;. J Biol Chem. 2014;289:27640-27652. (from Pfam)

Date:

2024-08-14

The SMF family, of DNA processing chain A, dprA, are a group of bacterial proteins. In H. pylori, dprA is required for natural chromosomal and plasmid transformation [1]. It has now been shown that DprA is found to bind cooperatively to single-stranded DNA (ssDNA) and to interact with RecA. In the process, DprA-RecA-ssDNA filaments are produced and these filaments catalyse the homology-dependent formation of joint molecules. While the E.coli SSB protein limits access of RecA to ssDNA, DprA alleviates this barrier. It is proposed that DprA is a new member of the recombination-mediator protein family, dedicated to natural bacterial transformation [2]. [1]. 10640603. The dprA gene is required for natural transformation of. Helicobacter pylori.. Smeets LC, Bijlsma JJ, Kuipers EJ, Vandenbroucke-Grauls CM,. Kusters JG;. FEMS Immunol Med Microbiol 2000;27:99-102.. [2]. 17803906. A key presynaptic role in transformation for a widespread. bacterial protein: DprA conveys incoming ssDNA to RecA.. Mortier-Barriere I, Velten M, Dupaigne P, Mirouze N, Pietrement. O, McGovern S, Fichant G, Martin B, Noirot P, Le Cam E, Polard. P, Claverys JP;. Cell. 2007;130:824-836. (from Pfam)

Date:

2024-08-14

DNA-protecting protein DprA protects incoming foreign DNA and is an accessory factor for RecA-mediated DNA strand exchange

Date:

2020-02-25

Gene:

dprA

Date:

2021-09-22

Disruption of this gene in both Haemophilus influenzae and Helicobacter pylori drastically reduces the efficiency of transformation with exogenous DNA, but with different levels of effect on chromosomal (linear) and plasmid (circular) DNA [1][2]. This difference suggests the DprA is not active in recombination, and it has been shown not to affect DNA binding, leaving the intermediate step in natural transformation, DNA processing. In Strep. pneumoniae, inactivation of dprA had no effect on the uptake of DNA [3]. All of these data indicated that DprA is required at a later stage in transformation. Subsequently DprA and RecA were both shown in S. pneumoniae to be required to protect incoming ssDNA from immediate degradation. [4]. Role of DprA in non-transformable species is not known. The gene symbol smf was assigned in E. coli, but without assignment of function.

Gene:

dprA

Date:

2021-04-27