This entry contains 2-phospho-L-lactate transferase (CofD), phosphoenolpyruvate transferase and related sequences. CofD catalyses the fourth step in the biosynthesis of coenzyme F420, which is the transfer of the 2-phospholactate moiety from lactyl (2)diphospho-(5') guanosine (LPPG) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of the L-lactyl phosphodiester of 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (F420-0) and GMP. F420 is a flavin derivative found in methanogens, Mycobacteria, and several other lineages. This enzyme is characterised so far in Methanocaldococcus jannaschii (Methanococcus jannaschii) [1] but appears restricted to F420-containing species and is predicted to carry out the same function in these other species. CofD monomer contains 12 beta- strands, seven of them form a Rossmann fold, and 13 alpha-helices [2]. [1]. 11888293. Characterization of the 2-phospho-L-lactate transferase enzyme. involved in coenzyme F(420) biosynthesis in Methanococcus. jannaschii.. Graupner M, Xu H, White RH;. Biochemistry. 2002;41:3754-3761.. [2]. 18252724. Molecular insights into the biosynthesis of the F420 coenzyme.. Forouhar F, Abashidze M, Xu H, Grochowski LL, Seetharaman J,. Hussain M, Kuzin A, Chen Y, Zhou W, Xiao R, Acton TB, Montelione. GT, Galinier A, White RH, Tong L;. J Biol Chem. 2008;283:11832-11840. (from Pfam)
GO Terms:- Molecular Function:
- LPPG:FO 2-phospho-L-lactate transferase activity (GO:0043743)
- Date:
- 2024-08-14