This entry represents the bifunctional nuclease (BFN) domain which is specific to bacteria and plant organisms. It has both RNase and DNase activities [1]. The dimer of the BFN domain forms a wedge, each monomer being a basic triangular shape. The BFN domain is composed of an eight-stranded, distorted beta-sheet consisting of a four-stranded antiparallel beta-sheet, and a four-stranded mixed beta-sheet. This domain can be found in M. tuberculosis Carbon monoxide resistance (Cor) proteins. Cor consists entirely of this domain with homologues in a variety of organisms, including most mycobacteria, Bacteroides sp., Chlamydia sp., Streptomyces sp., and Rhodococcussp. One of the homologs from Oryza minuta protein OmBBD was reported to exhibit DNase and RNAse activity in vitro [1]. OmBBD carries a C-terminal UvrB domain that is absent in the mycobacterial sequences. UvrB is one component of the UvrABC endonuclease system. Hence, it was proposed that OmBBD's observed nuclease activity may come not from this domain but from an interaction of the C-terminal UvrB with the catalytic UvrC nuclease [2]. [1]. 20018603. Novel bifunctional nucleases, OmBBD and AtBBD1, are involved in. abscisic acid-mediated callose deposition in Arabidopsis.. You MK, Shin HY, Kim YJ, Ok SH, Cho SK, Jeung JU, Yoo SD, Kim. JK, Shin JS;. Plant Physiol. 2010;152:1015-1029.. [2]. 24255121. cor, a novel carbon monoxide resistance gene, is essential for. Mycobacterium tuberculosis pathogenesis.. Zacharia VM, Manzanillo PS, Nair VR, Marciano DK, Kinch LN,. Grishin NV, Cox JS, Shiloh MU;. MBio. 2013;4:e00721-e00713. (from Pfam)
GO Terms:- Molecular Function:
- nuclease activity (GO:0004518)
- Date:
- 2024-08-14