Protein Family Models

Date:

2024-08-14

Date:

2024-08-14

This domain is responsible for the 3'-5' exonuclease proofreading activity of E. coli DNA polymerase I (polI) and other enzymes, it catalyses the hydrolysis of unpaired or mismatched nucleotides. This domain consists of the amino-terminal half of the Klenow fragment in E. coli polI it is also found in the Werner syndrome helicase (WRN), focus forming activity 1 protein (FFA-1) and ribonuclease D (RNase D). Werner syndrome is a human genetic disorder causing premature aging; the WRN protein has helicase activity in the 3'-5' direction [4,5]. The FFA-1 protein is required for formation of a replication foci and also has helicase activity; it is a homologue of the WRN protein [3]. RNase D is a 3'-5' exonuclease involved in tRNA processing. Also found in this family is the autoantigen PM/Scl thought to be involved in polymyositis-scleroderma overlap syndrome. [1]. 3883192. Structure of large fragment of Escherichia coli DNA polymerase I. complexed with dTMP.. Ollis DL, Brick P, Hamlin R, Xuong NG, Steitz TA;. Nature 1985;313:762-766.. [2]. 9396823. The proofreading domain of Escherichia coli DNA polymerase I and. other DNA and/or RNA exonuclease domains.. Moser MJ, Holley WR, Chatterjee A, Mian IS;. Nucleic Acids Res 1997;25:5110-5118.. [3]. 9697700. Replication focus-forming activity 1 and the Werner syndrome. gene product. Yan H, Chen CY, Kobayashi R, Newport J;. Nat Genet 1998;19:375-378.. [4]. 9288107. The Werner syndrome protein is a DNA helicase.. Gray MD, Shen JC, Kamath-Loeb AS, Blank A, Sopher BL, Martin GM,. Oshima J, Loeb LA;. Nat Genet 1997;17:100-103.. [5]. 9224595. DNA helicase activity in Werner's syndrome gen. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-08-14

Date:

2024-08-14

Date:

2024-08-14

DNA polymerase I is a family A polymerase which functions primarily to fill DNA gaps that arise during DNA repair, recombination and replication and it has two functional domains, a 5'-3' polymerase and 5'-3' exonuclease domain.

Date:

2022-08-04

Has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair

Gene:

polA

Date:

2021-07-21

All proteins in this family for which functions are known are DNA polymerases Many also have an exonuclease motif. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University).

Gene:

polA

Date:

2021-04-27