Protein Family Models

Date:

2024-08-14

YpeB is a sporulation protein that, together with the spore-cortex-lytic enzyme SleB, is required for normal germination. YpeB stabilises SleB enzyme and may regulate its activity during spore germination [1-6]. It consists of three tandemly repeated PepSY domains at the C-terminal, being the first two (PepSY1 and PepSY2) represented in this entry and the last one in Pfam:PF03413. [1]. 10658652. Complete spore-cortex hydrolysis during germination of Bacillus. subtilis 168 requires SleB and YpeB.. Boland FM, Atrih A, Chirakkal H, Foster SJ, Moir A;. Microbiology 2000;146:57-64.. [2]. 11567005. Two class A high-molecular-weight penicillin-binding proteins of. Bacillus subtilis play redundant roles in sporulation.. McPherson DC, Driks A, Popham DL;. J Bacteriol. 2001;183:6046-6053.. [3]. 16771673. Role of membrane-bound thiol-disulfide oxidoreductases in. endospore-forming bacteria.. Moller M, Hederstedt L;. Antioxid Redox Signal. 2006;8:823-833.. [4]. 16905870. Subcellular localization of a germiantion-specific cortex-lytic. enzyme, SleB, of Bacilli during sporulation.. Masayama A, Fukuoka H, Kato S, Yoshimura T, Moriyama M, Moriyama. R;. Genes Genet Syst. 2006;81:163-169.. [5]. 21112786. Germination of spores of Bacillales and Clostridiales species:. mechanisms and proteins involved.. Paredes-Sabja D, Setlow P, Sarker MR;. Trends Microbiol. 2011;19:85-94.. [6]. 26219275. Crystal structure of the PepSY-containing domain of the YpeB. protein involved in germination of bacillus spores.. Ustok F, Chirgadze D, Christie G.. Proteins. 2015;83(10):1914-21 (from Pfam)

Date:

2024-08-14

This region is likely to have an protease inhibitory function (personal obs:C Yeats). This model is likely to miss some members of this family as the separation from signal to noise is not clear. The name is derived from Peptidase & Bacillus subtilis YPEB. [1]. 7674922. Evolutionary families of metallopeptidases.. Rawlings ND, Barrett AJ;. Meth Enzymol 1995;248:183-228.. [2]. 15124630. The PepSY domain: a regulator of peptidase activity in the. microbial environment?.. Yeats C, Rawlings ND, Bateman A;. Trends Biochem Sci 2004;29:169-172.. [3]. 15583150. Identification of residues in the Pseudomonas aeruginosa. elastase propeptide required for chaperone and secretion. activities.. McIver KS, Kessler E, Ohman DE;. Microbiology 2004;150:3969-3977. (from Pfam)

Date:

2024-08-14

germination protein YpeB is required for spore cortex hydrolysis during germination

Date:

2020-05-01

Members of this family are YpeB, a protein usually encoded with the putative spore-cortex-lytic enzyme SleB and required, together with SleB, for normal germination. This family is retricted to endospore-forming species in the Firmicutes lineage of bacteria, and found in all such species to date except Clostridium perfringens. The matching phenotypes of mutants in SleB (called a lytic transglycosylase) and YpeB suggests that YpeB is necessary to allow SleB to function.

Gene:

ypeB

Date:

2024-06-27