Protein Family Models

This is the C-terminal domain found in GEN1 resolvase. It is composed of three-strand antiparallel beta sheets and four alpha helices [1]. GEN1 protein, a member of the XPG/Rad2 family of structure-selective endonucleases, is specialized for the cleavage of Holliday junction recombination intermediates [2]. Structural comparison indicates that the C-terminal domain is similar to a series of chromobox homology proteins [1]. Functional analysis indicates that the chromodomain provides an additional DNA binding site necessary for efficient HJ cleavage, and its truncation severely hampers GEN1's catalytic activity [3]. [1]. 26686639. Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to. DNA.. Liu Y, Freeman AD, Declais AC, Wilson TJ, Gartner A, Lilley DM;. Cell Rep. 2015;13:2565-2575.. [2]. 28049850. Resolution of single and double Holliday junction recombination. intermediates by GEN1.. Shah Punatar R, Martin MJ, Wyatt HD, Chan YW, West SC;. Proc Natl Acad Sci U S A. 2017;114:443-450.. [3]. 26682650. Human Holliday junction resolvase GEN1 uses a chromodomain for. efficient DNA recognition and cleavage.. Lee SH, Princz LN, Klugel MF, Habermann B, Pfander B,. Biertumpfel C;. Elife. 2015; [Epub ahead of print] (from Pfam)

Date:

2024-08-14

Members of this family are found in T4 RNaseH ribonuclease, and adopt a SAM domain-like fold, consisting of a bundle of four/five helices. These residues may have a role in providing a docking site for other proteins or enzymes in the replication fork [1]. [1]. 8674116. Structure of bacteriophage T4 RNase H, a 5' to 3' RNA-DNA and. DNA-DNA exonuclease with sequence similarity to the RAD2 family. of eukaryotic proteins.. Mueser TC, Nossal NG, Hyde CC;. Cell. 1996;85:1101-1112. (from Pfam)

Date:

2024-08-14

Rad2 family DNA repair protein, similar to DNA repair protein XPG (also known as ERCC5), a homolog of UVH3 and RAD2 proteins, which are involved in nucleotide excision repair (NER) and other DNA repair pathways

Date:

2023-06-05

XPG/RAD2 family endonuclease similar to Saccharomyces cerevisiae DNA repair protein RAD2, a single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents, and Homo sapiens Xeroderma pigmentosum group G-complementing protein (XPG), a single-stranded structure-specific DNA endonuclease involved in DNA excision repair

Date:

2019-03-21

XPG/RAD2 family endonuclease similar to Drosophila melanogaster mutant nucleotide excision repair protein mus201, a truncated XPG protein

Date:

2022-06-07

XPG/RAD2 family endonuclease similar to Saccharomyces cerevisiae DNA repair protein RAD2, a single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents, and Homo sapiens Xeroderma pigmentosum group G-complementing protein (XPG), a single-stranded structure-specific DNA endonuclease involved in DNA excision repair

Date:

2023-03-10

rad2 and TrwB_TraG_TraD_VirD4 domain-containing protein

Date:

2019-03-21

XPG/RAD2 family endonuclease similar to Saccharomyces cerevisiae DNA repair protein RAD2, a single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents, and Homo sapiens Xeroderma pigmentosum group G-complementing protein (XPG), a single-stranded structure-specific DNA endonuclease involved in DNA excision repair

Date:

2023-03-10

Rad2 family DNA repair protein, similar to DNA repair protein XPG (also known as ERCC5), a homolog of UVH3 and RAD2 proteins, which are involved in nucleotide excision repair (NER) and other DNA repair pathways; XPG/RAD2 family endonuclease similar to Saccharomyces cerevisiae DNA repair protein RAD2, a single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents, and Homo sapiens Xeroderma pigmentosum group G-complementing protein (XPG), a single-stranded structure-specific DNA endonuclease involved in DNA excision repair

Date:

2017-03-02

XPG/RAD2 family PIN (PilT N terminus) domain-containing protein similar to the PIN domain of XPG nuclease that plays a central role in nucleotide excision repair (NER) in cleaving DNA bubble structures or loops; XPG/RAD2 family endonuclease similar to Drosophila melanogaster mutant nucleotide excision repair protein mus201, a truncated XPG protein; XPG/RAD2 family endonuclease similar to Drosophila melanogaster mutant nucleotide excision repair protein mus201, a truncated XPG protein

Date:

2017-03-02

XPG/RAD2 family PIN (PilT N terminus) domain-containing protein similar to the PIN domain of XPG nuclease that plays a central role in nucleotide excision repair (NER) in cleaving DNA bubble structures or loops

Date:

2022-05-26

All proteins in this family for which functions are known are flap endonucleases that generate the 3' incision next to DNA damage as part of nucleotide excision repair. This family is related to many other flap endonuclease families including the fen1 family. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University).

Date:

2022-03-28