Single-Cell RNA-Sequencing of Human Prostatectomy Tissue

The tissue microenvironment in prostate cancer is profoundly altered. How prostate cancer cells and their precursors mediate those changes is unclear, in part due to the inability to longitudinally study the disease evolution in human tissues. To overcome this limitation, we performed extensive single-cell RNA-sequencing (scRNA-seq) to assess the transcriptional profiles of the tissue microenvironment in prostate tissues from prostatectomies from men diagnosed with prostate cancer. For each subject, benign-enriched and tumor-enriched tissues were collected from the peripheral zone of the prostate. Our studies of human tissues revealed that cancer cell-intrinsic activation of MYC signaling was the top up-regulated pathway in human cancers, representing a common denominator across the well-known molecular and pathological heterogeneity of human prostate cancer. Numerous non-malignant cell states in the tumor microenvironment (TME), including non-cancerous epithelial, immune, and fibroblast cell compartments, were conserved across individuals, suggesting that these cell types may be a sequelae of the convergent MYC activation in the cancer cells.

• Study Weblinks:
  • Preprint
• Study Design:
  • Tumor vs. Matched-Normal
• Study Type:
  • Single Cell Analysis
• Total number of consented subjects: 7
• Subject Sample Telemetry Report (SSTR)

• BioProject
• PubMed
• Sequence Read Archive
• BioSample
• MeSH
• Gene
• PMC

Men diagnosed with primary prostate cancer with no prior treatment who underwent prostatectomy.

• Primary Phenotype: Prostatic Neoplasms
• Tumor Microenvironment

• MYC

• Principal Investigator
  • Srinivasan Yegnasubramanian. Johns Hopkins University, Baltimore, MD, USA.
• Co-Investigator
  • Angelo De Marzo. Johns Hopkins University, Baltimore, MD, USA.
• Sequencing Center
  • Sidney Kimmel Comprehensive Cancer Center Experimental and Computational Genomics Core. Johns Hopkins University, Baltimore, MD, USA.
• First Author
  • Mindy Kim Graham. Northwestern University, Chicago, IL, USA.