Genetic Effects on miRNA Expression During Mid-Gestation Neocortical Development

In this study we aimed to find common genetic variants that influence miRNA expression during mid-gestation neocortical development. Small RNA-sequencing from 212 fetal cortical tissue samples between 14 and 21 weeks gestation were used to profile miRNA expression. Combined with donor genotypes, we were able to find miRNA-eQTLs during neocortical development. The dataset provided here includes 212 Small RNA-sequencing files in FASTQ format from cortical wall samples. Also provided here are 212 donor genotypes in PLINK format (hg19).

• Study Weblinks:
  • Effects of miR-4707-3p expression on differentiation of primary human neural progenitor cells
• Study Design:
  • Collection
• Study Type:
  • eQTL
  • RNA Sequencing
• dbGaP estimated ancestry using GRAF-pop
• Total number of consented subjects: 212
• Subject Sample Telemetry Report (SSTR)

• PubMed
• Sequence Read Archive
• BioSample
• MeSH
• Gene
• PMC

De-identified prenatal tissue samples were obtained from the UCLA Gene and Cell Therapy Core according to IRB guidelines. Full informed consent was obtained from all the parent donors. All genotyped donors that passed quality control (QC) were included in the study. QC steps included sufficient read depth, correction for sample swaps and no contamination, checked with verifyBamID.

Total RNA Sequencing was released under dbGaP Study Accession: phs001900.v1.p1

• Primary Phenotype: MicroRNAs
• Hypovolemia
• Educational Status

• MIR4707

• Principal Investigator
  • Jason L. Stein, PhD. University of North Carolina at Chapel Hill, NC, USA.
• Funding Sources
  • R01MH120125. National Institutes of Health, Bethesda, MD, USA.
  • R01MH118349. National Institutes of Health, Bethesda, MD, USA.
  • U54EB020403. National Institutes of Health, Bethesda, MD, USA.
  • R00MH102357. National Institutes of Health, Bethesda, MD, USA.