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- Study Description
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- Instructions for requestors
- Data Use Certification (DUC) Agreement
- Talking Glossary of Genetic Terms
Cancer-associated fibroblasts (CAFs) are major players in the progression and drug resistance of pancreatic ductal adenocarcinoma (PDAC). CAFs constitute a diverse cell population consisting of several recently described subtypes, although the extent of CAF heterogeneity has remained undefined. Here we employ single-cell RNA-sequencing to thoroughly characterize the neoplastic and tumor microenvironment content of human PDAC tumors. Six human PDAC tumor specimens from six patients were collected, and processed for single-cell RNA-sequencing analysis. Adjacent-normal pancreas tissue was also collected from two of the patients. Tumor samples were digested, and fluorescence-activated cell sorting was used to isolate viable cells. For one tumor sample, viable, CD45-negative, CD31-negative, and EpCAM-negative cells were also isolated to enrich for CAFs. The 10X Chromium platform was then used to isolate single cells for RNA-sequencing analysis. This work has demonstrated the differences in immune cell populations between adjacent-normal and tumor tissues, and identified subpopulations of epithelial cells and CAFs present in PDAC tumors. This high-throughput analysis is a resource to better understand the cell populations present in PDAC, and may ultimately aid in the development of more effective therapies for this deadly malignancy.
- Study Design:
- Prospective Longitudinal Cohort
- Study Type:
- Cohort
- Total number of consented subjects: 6
- Subject Sample Telemetry Report (SSTR)
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- Authorized Access
- Publicly Available Data
- Link to other NCBI resources related to this study
- Molecular Data
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Type Source Platform Number of Oligos/SNPs SNP Batch Id Comment Single-Cell RNA-sequencing Illumina HiSeq 4000 N/A N/A - Study History
Consenting pancreatic cancer patients were included in this study.
- Selected Publications
- Diseases/Traits Related to Study (MeSH terms)
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- Primary Phenotype: Carcinoma, Pancreatic Ductal
- Cancer-Associated Fibroblasts
- Tumor Microenvironment
- Authorized Data Access Requests
- Study Attribution
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Principal Investigator
- David Tuveson, MD, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA; Lustgarten Foundation Pancreatic Cancer Research Laboratory, Cold Spring Harbor, NY, USA.
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Co-Principal Investigator
- Paul Robson, PhD. The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA; 8Department of Genetics and Genome Sciences, Institute for Systems Genomics, University of Connecticut, Farmington, CT, USA.
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Other Investigators
- Ela Elyada, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA; Lustgarten Foundation Pancreatic Cancer Research Laboratory, Cold Spring Harbor, NY, USA.
- Mohan Bolisetty, PhD. The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA; Bristol-Myers Squibb, Pennington, NJ, USA.
- Pasquale Laise, PhD. Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA.
- William F. Flynn, PhD. The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
- Elise T. Courtois, PhD. The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
- Richard A. Burkhart, MD. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.
- Jonathan A. Teinor, MD. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.
- Pascal Belleau, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
- Giulia Biffi, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA; Lustgarten Foundation Pancreatic Cancer Research Laboratory, Cold Spring Harbor, NY, USA.
- Matthew Lucito. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA; Lustgarten Foundation Pancreatic Cancer Research Laboratory, Cold Spring Harbor, NY, USA.
- Santhosh Sivajothi. The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
- Todd D. Armstrong. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.
- Dannielle Engle, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA; Lustgarten Foundation Pancreatic Cancer Research Laboratory, Cold Spring Harbor, NY, USA; The Salk Institute, La Jolla, CA, USA.
- Kenneth H. Yu, MD. Memorial Sloan Kettering Cancer Center, New York, NY, USA.
- Yuan Hao, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
- Christopher L. Wolfgang, MD, PhD. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.
- Youngkyu Park, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA; Lustgarten Foundation Pancreatic Cancer Research Laboratory, Cold Spring Harbor, NY, USA.
- Jonathan Preall, PhD. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
- Elizabeth M. Jaffee, MD. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA.
- Andrea Califano, PhD. Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA.
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Funding Source
- The Lustgarten Foundation. Woodbury, NY, USA.
- P30CA045508. Bethesda, MD, USA.
- R01CA190092. Bethesda, MD, USA.
- U01CA224013. Bethesda, MD, USA.
- P30CA034196. Bethesda, MD, USA.
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Principal Investigator