High-Resolution Clonal Mapping of Multi-Organ Metastasis in Triple Negative Breast Cancer

Clonal dynamics during metastasis were studied in two patient-derived xenograft (PDX) models established from treatment-naive primary breast tumors from TNBC patients diagnosed with concurrent metastatic disease. Genomic sequencing and barcode-mediated clonal tracking revealed robust alterations in clonal architecture between primary tumors and metastases. Polyclonal seeding and maintenance of low-abundance subclones was observed in each metastatic lesion examined. Lung, liver, and brain metastases were enriched for an identical population of subclones. Clones dominating multi-organ metastases shared a genomic lineage. Thus, properties of rare mammary tumor subclones enabled the seeding and colonization of metastases in multiple secondary organ sites.

• Study Design:
  • Case-Control
• Study Type:
  • Case-Control
• Total number of consented subjects: 1
• Subject Sample Telemetry Report (SSTR)

Patient-derived xenograft models employed in this study were derived from two patients with treatment-naive triple negative breast cancer diagnosed with synchronous metastases.

• Primary Phenotype: Triple Negative Breast Neoplasms
• Neoplasm Metastasis

• Principal Investigator
  • Helen Piwnica-Worms, PhD. The University of Texas MD Anderson Cancer Center, Houston, TX, USA.