Neighbor-joining phylogenetic tree of MsHSP17.7 and sHSPs from other plant species. The phylogenetic tree was constructed based on similarities with 50 plant sHSPs, which divided the sHSP gene family into six clades. Amino acid sequences used in the analysis were retrieved from GenBank or EMBL. Their database accession numbers are as follows: AtHSP18.1 (Arabidopsis thaliana, P19037); NtHSP18.0 (Nicotiana tabacum, A0A068LKK5); PvHSP18.5 (Phaseolus vulgaris, T2DN13); DcHSP18.0 (Daucus carota, P27397); GmHSP18.5 (Glycine max, P05478); GmHSP17.3 (Glycine max, P02519); PtHSP18.2 (Populus trichocarpa, B9HHJ3); PsHSP18.1 (Pisum sativum, P19243); AtHSP17.6 (Arabidopsis thaliana, Q9ZW31); MtHSP17.6 (Medicago truncatula, Q2HTU2); MsHSP18.2 (Medicago sativa, P27880); StHSP17.6 (Solanum tuberosum, W5XNJ3); SlHSP17.8 (Solanum lycopersicum, P30221); SpHSP17.6 (Solanum peruvianum, O82012); StHSP (Solanum tuberosum, Q41218); AtHSP22.0 (Arabidopsis thaliana, Q38806); GmHSP22.0 (Glycine max, P30236); PsHSP 22.7 (Pisum sativum, P19244); PdHSP17.5 (Prunus dulcis, Q9XGS6); PlHSP (Prunus salicina, C9EIM5); CpHSP19 (Citrus paradisi, Q84LP5); TcHSP17.6 (Theobroma cacao, A0A061GJC5); JcHSP17.5 (Jatropha curcas, D5JG84); NtHSP (Nicotiana tabacum, Q53E18); NtHSP17.6 (Arabidopsis thaliana, A0A077DBK4); AmHSP (Ammopiptanthus mongolicus, S5TJ94); PsHSP17.1 (Pisum sativum, P19242); MtHSP (Medicago truncatula, G7J8C7); AlHSP17.7 (Arabidopsis thaliana, O81822); AtHSP17.6 (Arabidopsis thaliana, P29830); AtHSP17.6 (Arabidopsis thaliana, P29830); OsHSP16.0 (Oryza sativa, Q652V8); AtHSP15.7 (Arabidopsis thaliana, Q9FHQ3); GmHSP (Glycine max, B0M1A7); MtHSP (Medicago truncatula, G7KG40); TaHSP26.6 (Triticum aestivum, Q9SBB7); OsHSP26.7 (Oryza sativa, Q10P60); AsHSP26.8 (Agrostis stolonifera, Q8GV37); SaHSP (Spartina alterniflora, J7H8N1); PhHSP22 (Petunia hybrida, P30222); SlHSP21 (Solanum lycopersicum, Q95661); CaHSP (Capsicum annuum, D9IAX1); OsHSP24.1 (Oryza sativa, Q6Z7V2); AtHSP23.6 (Arabidopsis thaliana, Q96331); AtHSP23.5 (Arabidopsis thaliana, Q9FGM9); PsHSP22 M (Pisum sativum, P46254); TcHSP23.6 (Theobroma cacao, A0A061FZB7); PvHSP22 (Phaseolus vulgaris, V5N8V1); GmHSP22 (Glycine max, Q39818)