PMC

Phosphorimages of selectivity tests in the presence of 0.005 U/µl M184V and 50 nM hybrid P1/T4 (A), hybrid P2/T4 (B), hybrid P3/T4 (C) or hybrid P4/T4 (D). The concentration of building blocks is 10 µM. On the bottom of the resulting phosphorimages are indicated the triphosphate building blocks added as substrates. The left lane of the two columns represents the natural triphosphate building block, the right lane its anhydrohexitol analogue. (A) Lane 2, dTTP; lane 3, hTTP; lane 4, dGTP; lane 5, hGTP; lane 6, dTTP + dGTP; lane 7, hTTP + hGTP; lane 8, dTTP + dGTP + dATP; lane 9, hTTP + hGTP + hATP; lane 10, dTTP + dGTP + dATP + dCTP; lane 11, hTTP + hGTP + hATP + hCTP; lane 12, dATP; lane 13, hATP; lane 14, dCTP; lane 15, hCTP. (B) Lane 2, dGTP; lane 3, hGTP; lane 4, dATP; lane 5, hATP; lane 6, dGTP + dATP; lane 7, hGTP + hATP; lane 8, dGTP + dATP + dCTP; lane 9, hGTP + hATP + hCTP; lane 10, dGTP + dATP + dCTP + dTTP; lane 11, hGTP + hATP + hCTP + hTTP; lane 12, dCTP; lane 13, hCTP; lane 14, dTTP; lane 15, hTTP. (C) Lane 2, dATP; lane 3, hATP; lane 4, dCTP; lane 5, hCTP; lane 6, dATP + dCTP; lane 7, hATP + hCTP; lane 8, dATP + dCTP + dTTP; lane 9, hATP + hCTP + hTTP; lane 12, dGTP; lane 13, hGTP; lane 14, dATP; lane 15, hATP. (D) Lane 2, dCTP; lane 3, hCTP; lane 4, dTTP; lane 5, hTTP; lane 6, dCTP + dTTP; lane 7, hCTP + hGTP; lane 12, dGTP; lane 13, hGTP; lane 14, dATP; lane 15, hATP. Lane 1 represents the blank reaction in the absence of NTP and enzyme. The reaction time was 60 min.

The QoS parameter (transfer rate = T_f) of HTTP servers under normal flow without implementation of DASLM algorithm. (a) (Transfer rate = T_f) of HTTP server (s₁). (b) (Transfer rate = T_f) of HTTP server (s₂). (c) (Transfer rate = T_f) of HTTP server (s₃). (d) (Transfer rate = T_f) of HTTP server (s₄).

Missing referrer in HTTP request.

Complete HTTP request with referrer.