PMC

TDAG51 immunolocalisation in the primary cultures of elephant fibroblast cells after heat shock stimulus. (A) Cells without heat shock; (B) 0 h after heat shock; (C) 1 h after heat shock; (D) 2 h after heat shock; (E) 4 h after heat shock; (F) 6 h after heat shock; (G) 12 h after heat shock; (H) 18 h after heat shock; (I) 24 h after heat shock; (J) negative control. The scale bar represents 100 µm.

β-actin immunolocalisation in the primary cultures of elephant fibroblast cells after heat shock stimulus. (A) Cells without heat shock; (B) 0 h after heat shock; (C) 1 h after heat shock; (D) 2 h after heat shock; (E) 4 h after heat shock; (F) 6 h after heat shock; (G) 12 h after heat shock; (H) 18 h after heat shock; (I) 24 h after heat shock; (J) negative control. The scale bar represents 50 µm.

HSP70 immunolocalisation in the primary cultures of elephant fibroblast cells after heat shock stimulus. (A) Cells without heat shock; (B) 0 h after heat shock; (C) 1 h after heat shock; (D) 2 h after heat shock; (E) 4 h after heat shock; (F) 6 h after heat shock; (G) 12 h after heat shock; (H) 18 h after heat shock; (I) 24 h after heat shock; (J) localisation of HSP70 immunoexpression (upper; nucleus, middle; nucleus and cytoplasm, lower; cytoplasm); (K) negative control. The scale bar represents 50 µm (A–I,K), 20 µm (J).

HSP70 mRNA expression in As-treated seedlings on two consecutive days. (A) HSP70 mRNA expression in As-treated seedlings with heat shock. As1—at 0 h of heat shock; As2—after 15 min of heat shock; As3—after 30 min of heat shock; As4—after 45 min of heat shock; As5—after 1 h of heat shock; As6—after 1 h 15 min of heat shock; As7—after 1 h 45 min of heat shock; As8—after 2 h of heat shock; As9—after 2 h 15 min of heat shock; As10—after 2 h 30 min of heat shock; As11—after 2 h 45 min of heat shock; As12—after 3 h of heat shock. (B) HSP70 mRNA expression in As-treated seedlings in recovery for 3 h at ambient temperature after 3 h of heat shock. RAs1—at 0 h after withdrawal of heat shock; RAs2—at 15 min after withdrawal of heat shock; RAs3—at 30 min after withdrawal of heat shock; RAs4—at 45 min after withdrawal of heat shock; RAs5—at 1 h after withdrawal of heat shock; RAs6—at 1 h 15 min after withdrawal of heat shock; RAs7—at 1 h 45 min after withdrawal of heat shock; RAs8—at 2 h after withdrawal of heat shock; RAs9—at 2 h 15 min after withdrawal of heat shock; RAs10—at 2 h 30 min after withdrawal of heat shock; RAs11—at 2 h 45 min after withdrawal of heat shock; RAs12—at 3 h after withdrawal of heat shock. The graphs on the right represent data expressed as band intensities of HSP70 normalized to actin. The error bars are standard errors of the means of two measurements (n= 2) from band density measurements of the same gel. The data are representative of 2–3 experiments.