PMC

Corresponding ESI(+)-MS and MS/MS data is reported in . Compound 1. Ellagic Acid xylopyranoside or xylofuanoside Compound 2. Ellagic acid. Compound 3. Ellagic acid mannopyranoside. The configuration for each of the glycosylated ellagic acids could not be confirmed. Neutral loss of m/z 132 was used to confirm the presence of a pentose attached to ellagic acid.

Chromatograms of ellagic acid analysis. a A HPLC chromatogram of standard ellagic acid (EA) showing single peak of ellagic acid (arrow marked). b A HPLC chromatogram of pomegranate crude extract showing presence of ellagic acid (arrow marked). c A HPLC chromatogram of pomegranate extract, spiked with standard ellagic acid. Sample ellagic acid and standard ellagic acid co-eluted at the same retention time (arrow marked)

(A) Methanolic Soxhlet extract of the root of A. leiocarpa. (1) Gallic acid, (2) protocatechuic acid, (3a) flavonoid, (3b) alkaloid, (4a) flavonoid, (4b) ellagitannin, (5) aromadendrin, (6) di-galloyl-β-d-glucose, (7) ampelopsin, (8) methyltaxifolin, (9a*) flavonoid, (9b) isomer of methyltaxifolin, (9c) flavonoid, (10) ellagic acid derivatives, (11) taxifolin, (12) ellagic acid derivatives, (13) ellagic acid derivatives, (14) ellagic acid derivative, (15) di-methyl ellagic acid glucoside, (16) pentagalloylglucose, (17) ellagic acid derivatives, (18) di-methyl ellagic acid xylopyranoside, (19) ellagitannin, (20) ellagitannins, (21) di-methyl ellagic acid, (22a) ellagitannin, (22b) ellagitannin, (23) pinosylvin, (24) acetylated ellagic acid derivative, (25) ellagic acid derivatives, and (26) methylpinosylvin. (B) TLC chromatograms of RP₁₈ TLC fractions (Fr3–Fr8) obtained from the methanol Soxhlet extract (A); ** Unknown compounds; (EA der), ellagic acid derivatives; (Di-Me EA xyl), dimethyl ellagic acid xyloside; (ET), ellagitannin; (Ace-EA der), acetylated ellagic acid derivatives; and (PCA), protocatechuic acid. Main compounds with bold text.

HPLC chromatogram, mass spectrum, and schematic diagram of the reaction of ellagic acid and ellagic acid glucoside after column chromatography purification. Ellagic acid standard, after the reaction with glucansucrase, and purified ellagic acid glucoside (a), LC–MS/MS spectrum of ellagic acid glucoside (b), and a schematic diagram of the reaction (c)