PMC

All the TRV2 vectors were transformed into Agrobacterium strain GV3101 prior to its use for the treatment of S. pseudocapsicum seedlings. (A, E, I and M) CK, untreated S. pseudocapsicum seedlings. (B, F, J and N) TRV2 (mock), S. pseudocapsicum seedlings treated with TRV2. (C and G) Silenced S. pseudocapsicum seedlings treated with TRV2-SpPDS. The photobleached leaf phenotype observed in the PDS-silenced seedlings is shown in the first (C) and second (G) newly developed leaves. (K and O) Silenced S. pseudocapsicum seedlings treated with TRV2-SpChlH. The yellow-leaf phenotype observed in the ChlH-silenced seedlings is shown in the first (K) and second (O) newly developed leaves. (D, H, L and P) Silenced leaflets showing signs of the silencing phenotype along the vascular system. (D and H) Photobleached leaves of PDS-silenced seedlings. (L and P) Yellow leaves of ChlH-silenced seedlings. Photo credit: Hua Xu.

Bittersweet (Solanum dulcamara) (left) and Jerusalem cherry (Solanum pseudocapsicum) (right)

Amplification of SpACTIN, SpGAPDH, SpUBQ, SpPDS and SPChlH from S. pseudocapsicum leaves. The primers for SpACTIN, SpGAPDH and SpUBQ were designed from conserved regions of ACTIN, GAPDH and UBQ based on the alignment of tomato, pepper, tobacco and potato CDS sequences. The primers for SpPDS and SPChlH were designed from conserved regions of PDS and ChlH based on the alignment of tomato, pepper, and tobacco CDS sequences. The cDNA from S. pseudocapsicum leaves was used as the template for the amplification of the corresponding PCR products. Lane 1, RT-PCR products of the ACTIN gene; Lane 2, RT-PCR product of the GAPDH gene; Lane 3, RT-PCR product of the UBQ gene; Lane 4, RT-PCR product of the ChlH gene; Lane 5, RT-PCR product of the PDS gene; and Lane 6, 3′RACE product of the PDS gene. The gene-specific primers (GSPs) for 3′RACE were designed based on the sequence of Lane 5 according to the manufacturer’s instructions provided with the GeneRacer™ kit (Invitrogen, Carlsbad, CA, USA). All the primers are shown in .

Pseudocercospora solani-pseudocapsicicola (VIC 42807). a. Solanum pseudocapsicum with leaf spots; b. leaf spots on upper and lower leaf surface; c. close-up of lesion; d. close-up of lesion with fruiting; e, f. conidiophores emerging through stomata; g. conidiogenous cells; h–k. conidia. — Scale bars: e, f, h–k = 10 μm, g = 20 μm.