PMC

Comparison of the genomes of the FIM-1 positive P. aeruginosa strains. FI-14/157 was the FIM-1 index strain isolated in 2007. FI-17645 was the FIM-1 strain isolated in 2020. The core genomes differed by 16 SNPs. Differences in the accessory genome included the presence/absence of insertion sequences and prophages and the inversion of an ~21-kb region.

(a) Amino acid sequence alignment of FIM-1 with other acquired subclass B1 MBLs. Mature protein sequences are shown, either experimentally determined (for IMP-1, VIM-1, and SPM-1) (–) or deduced using the SignalP software. The sequence sources were as follows: FIM-1 (the present study), NDM-1 (GenBank/EMBL accession no. CAZ39946), DIM-1 (ADD91577), TMB-1 (CBY88906), GIM-1 (CAF05908), SIM-1 (AAX76774), KHM-1 (BAH16555), VIM-1 (CAC35170), IMP-1 (ADI87504), and SPM-1 (CAD37801). The positions are numbered according to the standard scheme proposed for class B β-lactamases (); the conserved zinc-binding residues typical of subclass B1 MBLs are indicated by black dots. (b) Phylogenetic tree of acquired subclass B1 MBLs, based on the sequences used for multiple alignment.

PCA biplot of the volatile components in the apple juice and cider samples. CK, apple juice; KM, the K. marxianus Fim-1 fermented cider; SC, the S. cerevisiae S288C fermented cider; AQ, the S. cerevisiae SY fermented cider.