PMC

Phylogenetic analysis of suborder Aspleniineae based on 74 chloroplast genes from 20 species. The phylogenetic tree was constructed using the maximum-likelihood method (ML) (Tamura et al. ). Numbers at the node are the bootstrap supporting value calculated from the ML method. For the Aspleniaceae family, the following sequences were used: Asplenium antiquum (OR764773; this study), Asplenium prolongatum (NC_035838.1; Wei et al. ), Asplenium nidus (NC_045119.1; Cui et al. ), Asplenium komarovii (MZ064529.1), Asplenium scolopendrium var. scolopendrium (MZ329815.1), Asplenium pekinense (NC_035837.1; Wei et al. ), Asplenium pseudocapillipes (NC_080354.1), Asplenium incisum (NC_071921.1; Kim et al. ), Asplenium castaneoviride (NC_071922.1; Kim et al. ), Asplenium ruprechtii (NC_071923.1; Kim et al. ), Hymenasplenium unilaterale (NC_035856.1; Wei et al. ). For the Rhachidosoraceae family, Rhachidosorus consimilis (NC_035862.1; Wei et al. ) is included. For the Athyriaceae family, the following species are included: Deparia viridifrons (NC_035846.1; Wei et al. ), diplazium maximum (MN623359.1; Liu et al. ), Diplazium dilatatum (NC_035850.1; Wei et al. ), Athyrium opacum (NC_035841.1; Wei et al. ), Athyrium spinulosum (NC_080880.1), Athyrium sinense (NC_035839.1; Wei et al. ), Athyrium brevifrons (OQ992626.1). As for an outgroup, Arabidopsis thaliana (KX551970.1; Stadermann et al. ) from Brassicaceae is included.

Cytological preparations of Asplenium centrafricanum, A. loxoscaphoides and A. theciferum: (A, B) Asplenium centrafricanum (EB392), meiosis with n = 70 bivalents; (C, D) Asplenium loxoscaphoides (RV7549), meiosis with n = 70 bivalents; (E, F) Asplenium theciferum (RV7219), meiosis with n = 70 bivalents (an enlargement of the central part of cell is shown in F); (A, C, E) photographs; (B, D, F) analytical drawings. Scale bars = 10 µm.

Results of genome size measurement using a flow cytometer. (A) Asplenium tenuicaule, (B) Asplenium sp.

Co-occurring Asplenium antiquum and Haplopteris zosterifolia.