(A) The specific expression patterns of genes in neo-tetraploid rice or autotetraploid rice. Upper lane: specific expressed genes in neo-tetraploid rice, a, c, e, g, i, k, m, o and q indicated the genes only expressed in autotetraploid parents, b, d, f, h, j. l, n, p, and r indicated the genes only expressed in neo-tetraploid rice lines. Middle lane: specific expressed genes in neo-tetraploid rice, a, c, e, g, i, k, m, o and q indicated the genes only expressed in neo-tetraploid rice lines, b, d, f, h, j. l, n, p, and r indicated the genes only expressed in autotetraploid parents. The lower lane indicated the expression of reference gene in neo-tetraploid rice lines and autotetraploid parents. (B) and (C) represent differentially expressed ncRNA in neo-tetraploid rice compared to autotetraploid rice, (B): the differentially expressed ncRNA in neo-tetraploid rice lines compared to T44, (C): the differentially expressed ncRNA in neo-tetraploid rice lines compared to T45. (D) and (E) represent the differentially expressed genes in neo-tetraploid rice lines compared to autotetraploid rice. (D): the differentially expressed genes in neo-tetraploid rice lines compared to T44, (E): the differentially expressed genes in neo-tetraploid rice lines compared to T45.