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| Status |
Public on Feb 07, 2019 |
| Title |
Activation of IGFBP7 Drives Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibition in Lung Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Previous study has demonstrated that HCC827/gef cells are resistant to gefitinib-induced aspoptosis. To investigate the regulators contributed to gefitinib resistance in lung cancer, we analyzed the gene expression profiles between HCC827 andHCC827/gef cells.
Reduced IGFBP7 in TKI-resistant cells reversed resistance to EGFR-TKIs, and increased EGFR-TKI-induced apoptosis through up-regulation of BIM and activation of caspases. Suppression of IGFBP7 attenuated phosphorylation of IGF-IR and downstream AKT in TKI-resistant cells.
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| Overall design |
RNAs extracted from gefitinib-sensitive HCC827 cells and gefitinib-resistant HCC827/gef cells were hybridized on Affymetrix microarrays. We tried to compare the differential gene expression profiles between HCC827 and HCC827/gef cells to identify the possible regulators in gefitinib resistance.
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| Contributor(s) |
Wu S, Chang T, Tsai M, Liu Y, Hsu C, Chang Y, Yu C, Shih J |
| Citation(s) |
30609749 |
| |
| Submission date |
Oct 31, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Tzu-Hua Chang |
| Organization name |
National Taiwan University Hospital
|
| Department |
Department of Internal Medicine
|
| Street address |
No. 7, Chung-Shan South Road
|
| City |
Taipei |
| ZIP/Postal code |
10002 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| GSM3452888 |
Gefitinib-sensitive lung cancer cell line,biological rep1 |
| GSM3452889 |
Gefitinib-sensitive lung cancer cell line,biological rep2 |
| GSM3452890 |
Gefitinib-sensitive lung cancer cell line,biological rep3 |
| GSM3452891 |
Acquired gefitinib-resistant lung cancer cell line,biological rep1 |
| GSM3452892 |
Acquired gefitinib-resistant lung cancer cell line,biological rep2 |
| GSM3452893 |
Acquired gefitinib-resistant lung cancer cell line,biological rep3 |
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| Relations |
| BioProject |
PRJNA503109 |
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