|
| Status |
Public on Feb 07, 2019 |
| Title |
Acquired gefitinib-resistant lung cancer cell line,biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
lung cancer cells, long term gefitinib treatment
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung adenocacinoma
|
| Treatment protocol |
HCC827/gef cells were selected from parental HCC827 cells that had been continuously exposed to increasing concentrations of gefitinib.
|
| Growth protocol |
Cells were cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS), 100 u/ml penicillin and 100 mg/ml streptomycin at 37 °C in a humidified atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)(Santa Clara, CA, USA, http://www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45°C on Affymetrix Human Genome U133 plus 2.0 GeneChip on Affymetrix Hybridization Oven 645, 60 RPM, then washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
The expression data were analyzed by the SAS JMP Genomics software (Version 4.0; SAS Institute Inc., Cary, NC). Affymetrix CEL files were transformed by RMA algorithm, imported at the probe level and log2 transformed, and each probe set was summarized using the median of the corresponding probes. Each array was normalized by Quantile Normalization.
|
| Description |
Gene expression data from the acquired gefitinib-resistant lung cancer cells
|
| Data processing |
RMA signal intensity
|
| |
|
| Submission date |
Oct 31, 2018 |
| Last update date |
Feb 07, 2019 |
| Contact name |
Tzu-Hua Chang |
| Organization name |
National Taiwan University Hospital
|
| Department |
Department of Internal Medicine
|
| Street address |
No. 7, Chung-Shan South Road
|
| City |
Taipei |
| ZIP/Postal code |
10002 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE122005 |
Activation of IGFBP7 Drives Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibition in Lung Cancer |
|
