|
| Status |
Public on Dec 03, 2012 |
| Title |
10_43_years_old_m |
| Sample type |
RNA |
| |
|
| Source name |
vastus laterlalis
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: muscles
age: 43 years old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Human quadriceps muscle samples were collected with the needle or by an open surgical procedure from anonymous healthy individuals that gave informed consent. Only age and gender are noted for those.
Total RNA was extracted from skeletal muscles using RNA Bee (Amsbio) according to the manufacturer's instructions. RNA integration number (RIN) was determined with RNA 6000 Nano (Agilent Technologies). RNA with RIN >7 were used for subsequent steps. RNA labeling was performed with the Illumina® TotalPrep RNA Amplification kit (Ambion) according to the manufacturer's protocol, and subsequently was hybridized to Illumina Human v3 Bead arrays.
|
| Label |
biotin
|
| Label protocol |
RNA labeling was performed with the Illumina® TotalPrep RNA Amplification kit (Ambion) according to the manufacturer's protocol, and subsequently was hybridized to Illumina Human v3 Bead arrays.
|
| |
|
| Hybridization protocol |
According to Illumina protocols
|
| Scan protocol |
According to Illumina protocols
|
| Description |
SAMPLE 10
|
| Data processing |
Microarray measurements were normalized using the quantile method. The quality of the data was assessed by principal component analysis (PCA). Human subjects were grouped into controls, pre-symptomatic, and symptomatic subjects. P-value cut-off of 0.05 were used due to high inter-individual variation. Probe annotation was done using the illuminaHumanv3BeadID package in R.
|
| |
|
| Submission date |
Sep 06, 2012 |
| Last update date |
Jan 26, 2015 |
| Contact name |
Marius Gheorghe |
| E-mail(s) |
marius.gheorghe@yahoo.com
|
| Organization name |
LUMC
|
| Department |
Human Genetics
|
| Street address |
Einthovenweg 20
|
| City |
Leiden |
| ZIP/Postal code |
2333 ZC Leiden |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE65314 |
