|
| Status |
Public on Sep 12, 2013 |
| Title |
Islets Nkx6.1 Mutant A |
| Sample type |
RNA |
| |
|
| Source name |
Isolated islets, mutant mice, pooled sample 1
|
| Organism |
Mus musculus |
| Characteristics |
strain: Nkx6.1-flox [mixed FVB/N x C57Bl/6J] X Pdx1-CreERTM [mixed Cd1 X C57Bl/6J background]
tissue: Pancreatic islets
gender: male
age: 6 weeks
treatment: Tamoxifen
genotype: Nkx6.1fl/-;Pdx1-CreERTM Mutant
|
| Treatment protocol |
Male mice were injected with 2 mg/25 g tamoxifen in corn oil four times between 4 and 6 weeks of age.
|
| Growth protocol |
All animal experiments described herein were approved by the University of California, Irvine and San Diego Institutional Animal Care and Use Committees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Islets were isolated and stored in 350 μl of RLT lysis buffer (QIAGEN, Valencia, CA) in the –80°C freezer. Each individual RNA sample was collected from two pancreata of the same genotype as per the manufacturer's instructions. We prepared RNA samples in ~12 μl of RNase free water. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
QuickAmp Low Input labelling: 150 ng total RNA labeled with Cy3. Specific Activity of > 6 pmol dye/µg cRNA accepted.
|
| |
|
| Hybridization protocol |
Standard Agilent hybridization with 1650 ng cRNA. 17-19 hrs @ 65°C. Standard Agilent wash including Stabilization & Drying Solution.
|
| Scan protocol |
Model G2505B Agilent Scanner. One color. 100% and 10% PMT settings for Extended Dynamic Range. 5µm resolution.
|
| Description |
Gene expression of islets at 6 weeks of age after deletion of Nkx6.1
|
| Data processing |
Raw Data extraction using Agilent Feature Extraction Software v10.7.3.1 using protocol GE1_107_Sep09 . Normalization, a modified LOESS.
|
| |
|
| Submission date |
Aug 29, 2012 |
| Last update date |
Sep 12, 2013 |
| Contact name |
Brandon Taylor |
| Organization name |
University of California, San Diego
|
| Department |
Pediatrics and Cellular & Molecular Medicine
|
| Lab |
Maike Sander, M.D.
|
| Street address |
Sanford Consortium for Regenerative Medicine, 2880 Torrey Pines Scenic Dr., Room 3101
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL10333 |
| Series (1) |
| GSE40470 |
Identification of Nkx6.1 regulated genes in mature pancreatic islets. |
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