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Sample GSM983150 Query DataSets for GSM983150
Status Public on Aug 10, 2012
Title Tumor_1031_HCI
Sample type RNA
Source name Secondary tumor 1031 regenerated from primary tumor 16 tissue, EF1α-PyMT10C lentivirus, adenocarcinoma histology
Organism Mus musculus
Characteristics tissue type: mouse mammary tumor
lentivirus: EF1α-PyMT10C
primary tumor: 16
histology: Adenocarcinoma
number of insertion sites: Unknown
combat group: 3
Treatment protocol No treatments
Growth protocol Mammary epithelial cells were infected with specified lentivirus, then transplanted into cleared fat pads of recipient mice. Tumors were harvested at 2 cm and a portion was flash frozen.
Extracted molecule total RNA
Extraction protocol Qiagen Rneasy kit
Label Cy3
Label protocol Agilent One-Color Quick Amp Labeling Protocol
Hybridization protocol Agilent One-color Gene Expression Hybridization and Wash Protocol
Scan protocol Microarrays were scanned on an Agilent Technologies G2505C scanner at 5 micron resolution
Data processing Array images were processed in Agilent Feature Extraction software version, which produces spatially detrended intensity data
Array data was filtered to remove control features and any non-uniform features or population outlier features. Remaining features were averaged to yield a single value for each unique probe. Finally data were log2 transformed, quantile normalized, and adjusted for batch effects using ComBat.
Submission date Aug 09, 2012
Last update date Feb 07, 2013
Contact name Bryan Welm
Phone 801-587-4633
Organization name University of Utah, Huntsman Cancer Institute
Department Oncological Science
Lab Bryan Welm Lab
Street address 2000 Circle of Hope Drive
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
Platform ID GPL7202
Series (1)
GSE40001 Targeting PyMT oncogene to diverse mammary cell populations enhances tumor heterogeneity and generates rare breast cancer subtypes

Data table header descriptions
VALUE Log 2 transformed normalized intensity data

Data table
A_51_P100021 4.496252379
A_51_P100034 10.81972818
A_51_P100052 4.677725049
A_51_P100084 6.485303718
A_51_P100099 7.783653243
A_51_P100155 10.338903
A_51_P100174 7.089560268
A_51_P100218 3.508701104
A_51_P100227 9.595831681
A_51_P100238 3.094771914
A_51_P100246 8.716379653
A_51_P100289 9.42992293
A_51_P100327 6.384486488
A_51_P100347 3.327584921
A_51_P100470 6.394464513
A_51_P100537 4.789252207
A_51_P100565 7.924990562
A_51_P100624 2.560100222
A_51_P100625 6.250384727
A_51_P100768 5.613116109

Total number of rows: 13988

Table truncated, full table size 340 Kbytes.

Supplementary file Size Download File type/resource
GSM983150_Tumor_1031_HCI.txt.gz 9.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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