|
| Status |
Public on Aug 10, 2012 |
| Title |
Tumor_96_HCI |
| Sample type |
RNA |
| |
|
| Source name |
Secondary tumor 96 regenerated from primary tumor 17 tissue, EF1α-PyMT10C lentivirus, adenocarcinoma histology
|
| Organism |
Mus musculus |
| Characteristics |
tissue type: mouse mammary tumor
lentivirus: EF1α-PyMT10C
primary tumor: 17
histology: Adenocarcinoma
number of insertion sites: 1- same site as tumor 17
combat group: 4
|
| Treatment protocol |
No treatments
|
| Growth protocol |
Mammary epithelial cells were infected with specified lentivirus, then transplanted into cleared fat pads of recipient mice. Tumors were harvested at 2 cm and a portion was flash frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit
|
| Label |
Cy3
|
| Label protocol |
Agilent One-Color Quick Amp Labeling Protocol
|
| |
|
| Hybridization protocol |
Agilent One-color Gene Expression Hybridization and Wash Protocol
|
| Scan protocol |
Microarrays were scanned on an Agilent Technologies G2505C scanner at 5 micron resolution
|
| Data processing |
Array images were processed in Agilent Feature Extraction software version 10.5.1.1, which produces spatially detrended intensity data
Array data was filtered to remove control features and any non-uniform features or population outlier features. Remaining features were averaged to yield a single value for each unique probe. Finally data were log2 transformed, quantile normalized, and adjusted for batch effects using ComBat.
|
| |
|
| Submission date |
Aug 09, 2012 |
| Last update date |
Feb 07, 2013 |
| Contact name |
Bryan Welm |
| E-mail(s) |
Bryan.Welm@hci.utah.edu
|
| Phone |
801-587-4633
|
| Organization name |
University of Utah, Huntsman Cancer Institute
|
| Department |
Oncological Science
|
| Lab |
Bryan Welm Lab
|
| Street address |
2000 Circle of Hope Drive
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE40001 |
Targeting PyMT oncogene to diverse mammary cell populations enhances tumor heterogeneity and generates rare breast cancer subtypes |
|
