|
Status |
Public on Aug 10, 2012 |
Title |
Tumor_63_HCI |
Sample type |
RNA |
|
|
Source name |
Primary tumor 63 generated from EF1α-PyMT10C lentivirus, tubular histology
|
Organism |
Mus musculus |
Characteristics |
tissue type: mouse mammary tumor lentivirus: EF1α-PyMT10C primary tumor: N/A histology: Tubular number of insertion sites: Unknown combat group: 4
|
Treatment protocol |
No treatments
|
Growth protocol |
Mammary epithelial cells were infected with specified lentivirus, then transplanted into cleared fat pads of recipient mice. Tumors were harvested at 2 cm and a portion was flash frozen.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen Rneasy kit
|
Label |
Cy3
|
Label protocol |
Agilent One-Color Quick Amp Labeling Protocol
|
|
|
Hybridization protocol |
Agilent One-color Gene Expression Hybridization and Wash Protocol
|
Scan protocol |
Microarrays were scanned on an Agilent Technologies G2505C scanner at 5 micron resolution
|
Data processing |
Array images were processed in Agilent Feature Extraction software version 10.5.1.1, which produces spatially detrended intensity data Array data was filtered to remove control features and any non-uniform features or population outlier features. Remaining features were averaged to yield a single value for each unique probe. Finally data were log2 transformed, quantile normalized, and adjusted for batch effects using ComBat.
|
|
|
Submission date |
Aug 09, 2012 |
Last update date |
Feb 07, 2013 |
Contact name |
Bryan Welm |
E-mail(s) |
Bryan.Welm@hci.utah.edu
|
Phone |
801-587-4633
|
Organization name |
University of Utah, Huntsman Cancer Institute
|
Department |
Oncological Science
|
Lab |
Bryan Welm Lab
|
Street address |
2000 Circle of Hope Drive
|
City |
Salt Lake City |
State/province |
UT |
ZIP/Postal code |
84112 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (1) |
GSE40001 |
Targeting PyMT oncogene to diverse mammary cell populations enhances tumor heterogeneity and generates rare breast cancer subtypes |
|